The Isolation of the Mitotic Apparatus from Mammalian Cells in Culture

Prev ious work h a s sugges ted t h a t s o m e pro te in(s ) assoc ia ted w i t h t he f u n c t i o n i n g o f t he mi to t i c a p p a r a t u s ( M A ) in h u m a n a m n i o n cells is synthes ized d u r i n g a re la t ive ly shor t pe r iod j u s t p r io r to t he e n t r y of cells in to divis ion, a n d t h a t th is p ro t e in is conse rved a n d reu t i l i zed by d a u g h ter cells d u r i n g s u b s e q u e n t d iv is ions (1). M o r e d i rec t ev idence in s u p p o r t o f th is conc lus ion shou ld be o b t a i n a b l e by b i o c h e m i c a l ana lys i s o f i sola ted M A ' s . A m e t h o d for i so la t ing M A ' s f r om c u l t u r e d m a m m a l i a n cells is t he sub jec t of th is repor t .